Sugar in the sample was quantified by comparing peak areas of the samples with those of the sugar standards such as fructose, glucose and sucrose.
Therefore bromelain activity will not be affected because according to Ngampanya and Phongtongpasuk,  sucrose concentration could affect on protein content, crude bromelain activity and specific activity of induced shoots of pineapple var.
The clear supernatant was collected and used for analysis. This is based on Gautam et al. In this study, the waste product of pineapple peelings becomes recyclable. Hence, alternatives to its efficient utilization are necessary .
The stem continues to grow and acquires at its apex a compact tuft of stiff, short leaves called the "crown" or "top".
It appears that once pineapple plants have been damaged by nematodes, they derive little benefit from nematicidal treatments. Tensile strength obtained will be referred for further study of natural fiber composites. This apotheosis of a luxuriant nature - marine and earthly - will find parallels in other exquisite pieces which, though apparently detached from those, share the same symbolic meaning.
The chemical compositions and morphology study of pineapple leaf and cassava indicate pineapple leaf have a high percentage to be used as an alternative pulp in paper making industry, promoting the green technology.